FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15033872
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT452280.3008406616738522No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN233860.1555554018286174No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC222830.14821863589100664No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG209660.1394584176318649No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA208220.1385005805556945No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA175340.11662996731647043No Hit
GGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGC170080.11313120132990356No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG164070.10913356186616462No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG161400.10735757228743201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC103700.047.1757245
GCGATTT101300.045.88962694
CACCCGG113200.043.4658664
TCACCCG116000.042.821533
CGGCCCG125750.039.4270258
CCAGTAG64500.030.5281815
CCGGCCC170400.029.5921657
CTCGATT96600.028.8172548-49
CCCGGCC181150.027.8859126
TAGCATA72350.027.6706339
GTTGACT85950.027.6664031
ATTCCGT99900.027.39344652-53
CGGACAC104700.027.35778212-13
GTAGCAT75400.027.0500378
CGGACAG105650.026.86753318-19
ACGGACA97300.026.15447218-19
GACACGG111200.025.7166714-15
TTCTCGA109650.025.6653746-47
TCTCGAT99350.025.39275448-49
CGTTCTT107950.025.29323876-77
Produced by FastQC (version 0.11.5)