FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14994308
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA227770.1519043092885647No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG189800.12658136674263326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT24150.033.9069331
GTATCGA21000.032.453084
CCCGTCG26200.029.278091
CGTATCG52250.018.8004043
CCGTCGG37350.018.624232
TATCGAA40600.018.1752685
TACGAGC9400.018.0000886
CCGTATC56550.017.8695262
CCCGGTT15600.017.7985171
TAAGCCG70800.016.79499866-67
GTCGAAT17550.016.3572061
ATCGCGT10350.016.34768994
GACGAAA73300.016.06231146-47
TCGACGA73850.016.0063344-45
AGTTTAC179100.015.666532594
ATTCGCA34500.015.39407292-93
TCGACCG8150.015.28179830-31
CTTACTT92050.015.1841011
TGTCGAG10350.014.985835
TGAACTC201800.014.975298
Produced by FastQC (version 0.11.5)