FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14334030
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA319510.2229031193600125No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA223440.15588079556133203No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG163640.11416189306147678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT55700.034.5650371
GTATCGA53650.033.7274634
TATCGAA61100.030.230415
CGGTTCG9450.025.3647773
CGTATCG88700.024.321023
CCGTATC92450.023.4361742
CCCGTCG23000.022.9223461
ATCGAAG91400.022.0598356
AGTTTAC173650.021.65234294
CGCGTAT2151.1783686E-619.7048421
TCGAAGG107250.019.1942447
TTGCACG52000.019.0709938
TACGACG12650.018.2053819
ACGGTTA35300.017.37520812-13
CCGTTAA36350.016.87313530-31
GCTACCT68400.016.5855481
TCTACGG49350.015.7141547
CGAAGGC122150.015.5446928
CGACACG24550.015.3155013
TATCGCG4450.015.31431986-87
Produced by FastQC (version 0.11.5)