FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14334030
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA366150.2554410727478595No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA310940.2169243401890466No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC270230.18852339502568363No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT244270.17041264738527825No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN211310.1474184161746557No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT203260.14180241006890595No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG202560.14131406171188424No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG186240.12992856858817792No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC176700.12327307812248195No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC164250.11458745377259572No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT143450.10007653116395039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT62650.036.8361194
CGACCGT23100.036.2139431
ACCCGGC71500.034.5741925
CACCCGG72700.034.2622074
GTAGGTC51950.031.5736162
TCACCCG80600.031.3121663
GTTGACT106600.031.21361
CGGCCCG88350.028.245438
ACCGTTG30250.027.654673
GACCGTT31500.026.7071152
TTGACTA122150.026.317652
CTCGATT54950.025.5863448-49
GACTATT131550.024.6865884
CCAGTAG56200.024.669375
CCGTCAT74050.024.3710331
ATTCCGT58850.024.08905252-53
TAGGTCT69500.023.8706233
AGTAGGT70150.023.4481281
CGGACAC62750.023.29204412-13
ACATGCC131750.022.7947442
Produced by FastQC (version 0.11.5)