FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14299080
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA315440.22060160513823265No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA225130.15744369567832336No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG162560.1136856357192211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT51900.033.552161
GTATCGA49750.032.3117034
TATCGAA56800.028.6321935
CGGTTCG10500.025.0683383
CGTATCG85350.024.3964713
CCGTATC89950.023.5668852
CCCGTCG21050.021.463731
AGTTTAC170800.020.94050494
ATCGAAG87000.020.6370746
TTGCACG53150.019.7193228
ACGGTTA34850.018.34114612-13
TCGAAGG101150.017.9818947
CCGGTAA14200.017.5660291
CCGTTAA36550.017.55230330-31
TGCACGG64600.016.5879489
TCGACCG12000.016.4496630-31
CGACACG22300.016.229783
GGTACCG40600.016.14873720-21
ACGACTT17400.015.6879011
TTCGCGC19800.015.6663967
Produced by FastQC (version 0.11.5)