FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14319241
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA369410.25798155083778535No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA311110.21726710235549498No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC270760.18908823449511045No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT246840.17238343847973508No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN224800.15699156121473198No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG205280.14335955376405773No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT204890.14308719295945924No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG185640.12964374298889167No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC178030.12432921549403352No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC164950.11519465312442188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT23350.039.054631
GCGATTT64000.036.57307494
ACCCGGC70300.035.4342735
CACCCGG72700.034.266544
GTTGACT109450.031.4808081
TCACCCG81950.031.1456893
GTAGGTC51050.030.3861662
ACCGTTG30400.029.8421943
GACCGTT31500.028.800792
CGGCCCG87500.028.6802548
CTCGATT55250.026.67758648-49
TTGACTA128000.026.5513132
GACTATT132100.026.0813434
ATTCCGT57150.025.17304852-53
CCGTCAT72450.024.9792861
CCAGTAG56700.024.7851245
AGTAGGT64150.024.5474191
CGGACAC63800.023.5707712-13
TTCTCGA64800.023.1068546-47
TAGGTCT68700.022.9895023
Produced by FastQC (version 0.11.5)