FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16090990
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGG375580.23341012579089293No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA333260.20710969306425522No Hit
GTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGA251520.1563110784358203No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA211650.13153323692327196No Hit
GTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGA171470.10656274101220621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT47450.032.5377271
GTATCGA49100.031.0166264
TATCGAA53300.028.7489135
CGCGTAT1955.966285E-1026.5526391
TAGGTAT84200.025.62063294
TCTACGG166450.023.238427
GGTCTAC167300.023.1786985
CGTATCG79600.022.4389023
TACGGAG174700.022.114119
CCGTATC82150.021.3418642
ATCGCGT11950.021.23807194
ATCGAAG96100.019.9548116
CTACGGA196050.019.8257318
GATGCGA200200.019.43817394
GTTAGGT212300.019.067691
AGGTCTA201100.019.0258224
TCAATCG48800.018.3951848
TTAACGG112500.018.2165034
CTTCGCG17850.018.168616
ACGTTCG11900.018.168616
Produced by FastQC (version 0.11.5)