FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16168418
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA494410.305787492629149No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN382890.23681352127338617No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG321300.19872074064388986No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC265600.16427086434801474No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC265560.16424612476001055No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT238990.147812853428208No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT207120.12810158668584645No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG187290.11583693593275482No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA173000.10699871811824756No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC164490.10173537077034994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT36300.050.762591
ACCGTTG44250.042.066153
GACCGTT45450.041.060192
GCGATTT51550.037.3836694
GTTGACT140250.034.522221
CCGTTGA57200.032.7883534
TTGACTA144250.032.6198962
CACCCGG67150.031.6399064
ACCCGGC69650.030.4349525
GTCTAAC30650.029.139861
GACTATT179850.026.318544
ACGTGCG34100.026.046818
CTCGATT47450.025.96057148-49
GTAGGTC37300.025.5830042
TCACCCG96000.025.559373
CGTTGAC76400.024.8545075
ATTCCGT49250.024.3424952-53
AACACGT37650.023.9691935
CGGCCCG89000.023.8668758
CGGACAC56000.021.98630712-13
Produced by FastQC (version 0.11.5)