FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004828332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12989075
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT563890.4341263715853515No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG299350.23046290825174234No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT258840.19927516008645726No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT250870.19313923431807115No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGGCCCCTAACAGGGGCC238490.1836081476163622No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN230540.17748761940322924No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT203370.15657004059180504No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT197080.15172750946468475No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT193570.14902523851775434No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA179810.13843172050357705No Hit
CCCAAACCCACCCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC172870.1330887688307289No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC138870.10691292490034895No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT137460.10582739725500084No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT135570.10437232828357677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)