FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13447015
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA173780.1292331420765129No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT165670.12320206380375125No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT165460.12304589531580057No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA164950.12266662898792037No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG164660.12245096774265517No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG153180.11391375706801844No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA143510.10672257002762324No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG141780.10543603915069627No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT136940.10183672733316651No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG134840.10027504245365979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT26100.035.3691021
GTATCGA24750.031.1418484
GTTACGA19750.029.983284
TTACGAC20000.029.6084945
TGTCGAG19050.028.864675
TGTTACG20550.028.3586543
CGTACGC55350.025.7276865
GTCGAGG21450.025.6350566
CCGTACG56050.025.3225274
ACGACTT41900.024.9544181
TCCGTAC59650.024.1094173
GTACGCC59750.023.8330966
TACGCCA60350.023.674027
TATCGAA35450.023.3330845
TACGACT26300.022.5159636
TTCCGTA68350.021.6622072
CCCGTCG66700.020.9013521
CGCGTAT2057.421422E-720.6774581
TGTGTCG27350.020.6205223
CGTATCG81700.020.4212343
Produced by FastQC (version 0.11.5)