FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13712525
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT255560.18636976049268825No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA251400.18333603767358675No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG205020.14951294528177705No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA201840.14719389754986772No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA201080.14663965972714726No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA200210.14600520327219094No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT196730.14346737745236565No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG184050.13422035693645043No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG166770.12161873907249031No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT158800.11580653453685591No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG153240.11175184730747983No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG144670.1055020865960135No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT142120.10364247284872773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT24000.038.6658361
TGTCGAG22950.037.4746255
GTCGAGG25150.034.1965266
GTATCGA22850.033.9364664
TTACGAC19350.031.8169985
GTTACGA19150.031.1676334
TGTTACG19550.030.5299323
TTCGCGC35150.029.9496387
ACGACTT42300.028.6197091
TGTGTCG31550.027.110713
TATCGAA30650.026.3734445
CCCGTCG74050.025.8905811
CGTACGC74650.025.8120755
CCGTACG75200.025.6857854
TCCGTAC77250.025.2475053
GTACGCC78250.024.6846146
TACGCCA78700.024.6031887
TACGACT25300.023.9628286
TTCCGTA85200.023.0610162
CGCCGCT44950.021.9562663
Produced by FastQC (version 0.11.5)