FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13520478
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT540950.4000968013113146No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT397920.2943091213195273No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT333560.24670725398909713No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG319930.23662624945656507No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT304960.22555415570366671No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT245590.1816429862908693No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC233140.17243473196731654No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC226260.16734615447767454No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT215470.15936566739726213No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT208890.1544989755539708No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT207630.153567055839298No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN190380.14080863117413453No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA182520.134995227239747No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG161970.11979606046472618No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC155030.11466310584581405No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT150930.11163066867902155No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC150180.11107595456314488No Hit
CAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTT140230.10371674729251436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT24250.058.5464631
ACCCGGC99650.054.717145
CACCCGG111650.049.089724
TCACCCG113650.048.603313
GACCGTT29800.047.1694032
ACCGTTG30850.046.172663
CGGCCCG119900.045.585938
GCGATTT115750.044.54091694
CCAGTAG78500.042.0349125
CTACCGT63700.036.5942238
TAGCATA95650.035.03289
CGCAAAA51300.034.1819531
GTAGCAT98450.033.9409648
ACTACCG69900.033.8867657
CGGACAC95850.033.14583212-13
CCCGGCC168000.033.0952076
CTCACCC166450.032.9604032
CCGGCCC173200.032.7247357
AGTAGCA109100.030.8435977
CGGACAG103250.030.6126918-19
Produced by FastQC (version 0.11.5)