FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13593674
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG382420.2813220325866282No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT200930.1478114010973045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT32800.031.1605951
GTATCGA29450.030.9596444
CGTACGC23900.029.1033745
CCGTACG25800.027.1422674
GTTACGA11150.026.5549344
GTACGCC27550.025.4181656
TACGCCA27750.025.4043357
ACGACTT26500.025.2384281
TTACGAC12050.024.5715755
CGTATCG54200.024.1927053
CCGTATC56400.023.9182112
TCCGTAC30150.023.38213
CCCGTCG39200.023.069331
TATCGAA41650.022.6809165
GAAATAA164500.022.57163894
GACGAAA114650.021.39837546-47
TCGACGA115100.021.23289144-45
TCGACCG31550.020.63145830-31
TAAGCCG119300.020.56420966-67
CGAAACA119700.020.55453548-49
Produced by FastQC (version 0.11.5)