FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11671385
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG130350.11168340346925408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT23400.037.6423571
GTATCGA21350.035.8810234
TATCGAA28700.028.820795
ACGACTT25600.025.7596051
CGTACGC27550.025.7590855
CCGTACG27850.025.481614
CGTATCG51150.023.7973843
TACGCCA30850.023.4606847
GTACGCC30850.023.3083426
TCCGTAC31900.022.8357943
CCGTATC55050.022.4558412
GTTACGA10950.022.3184644
TGTCGAG13750.021.5334155
TTACGAC12100.020.974115
ATCGAAG60000.020.8355756
TCGACCG29850.020.46868530-31
GTTCGAC30600.019.96695928-29
TTCCGTA40550.019.3578242
CGATCCG31250.019.25043782-83
CCCGTCG28800.017.8272821
Produced by FastQC (version 0.11.5)