FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11863811
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT415830.3505028864670889No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG214760.1810210901033403No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN194160.163657361028425No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT159880.13476276720861452No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT158860.1339030097495653No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT133200.11227420935819021No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG118830.1001617439792323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC87850.049.6182945
CACCCGG90650.047.9843754
TCACCCG93800.046.5247343
CGGCCCG103200.042.353278
GCGATTT97050.041.2588294
CGACCGT15050.039.37151
CCCGGCC133200.033.132226
CCAGTAG63050.032.5166135
CCGGCCC136300.032.3441857
TAGCATA66300.032.1123479
GTAGCAT68950.030.809738
CTCACCC144400.030.4179132
CGGACAC84550.029.73883212-13
ACCGTTG20400.029.2760373
GACCGTT20650.028.2391342
CTCGATT89700.028.1186248-49
CGCAAAA43050.027.9649941
GACACGG92150.027.64216614-15
CGGACAG91250.027.60782218-19
CTACCGT37400.027.3948298
Produced by FastQC (version 0.11.5)