FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11754325
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT411550.3501264428199833No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN222790.1895387442494571No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG209750.17844495536749239No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT158220.1346057727687468No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT155590.13236829847736897No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT128440.109270417484628No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG120500.10251545707643782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC89500.048.0122835
CACCCGG94000.045.664984
TCACCCG94300.045.5198973
GCGATTT96200.040.79224494
CGGCCCG105350.040.6919068
CGACCGT12700.037.7584841
CCAGTAG62250.033.0798845
CCCGGCC135100.032.1816376
TAGCATA65700.031.5506259
CCGGCCC141100.030.8826987
GTAGCAT68000.030.6226318
CTCACCC146400.029.6099472
CGGACAC83500.029.58092312-13
CTCGATT87550.027.78943648-49
CTACCGT40550.027.3570218
CGGACAG91150.027.1741118-19
GACACGG91550.027.10747314-15
ATTCCGT90200.026.94855352-53
ACCGTTG19000.026.724673
CCCGGAC97200.025.60546110-11
Produced by FastQC (version 0.11.5)