FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11201566
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT436910.39004367782147603No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG202190.18050154773002275No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT188040.16786938540557633No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN162190.1447922549400682No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT142930.1275982304617051No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC139960.12494681547205096No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT120930.10795811942723008No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT120330.10742248003538077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG90550.049.7871863
ACCCGGC93200.048.0624735
CACCCGG93650.047.8832174
CGACCGT16150.045.1176641
CGGCCCG109300.041.451058
GCGATTT102350.038.20446494
GACCGTT20450.035.6308172
CTACCGT38200.035.3099568
ACCGTTG21000.035.145343
CTCACCC133600.034.0609172
CCAGTAG55900.033.213485
TAGCATA58100.032.1946459
CCCGGCC146550.031.3962256
GTAGCAT61650.030.7220788
CGGACAC84500.030.39596712-13
CCGGCCC154850.030.1079397
CGCAAAA34850.028.8667851
GACACGG93000.027.92130714-15
CGGACAG92650.027.85060918-19
CTCGATT92600.027.51526848-49
Produced by FastQC (version 0.11.5)