FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11255320
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT432550.38430715430569723No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG198610.17645877682731367No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT187680.1667478134784262No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN185940.16520187786753288No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT146630.13027617162372995No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC140680.12498978260946823No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT121580.10802002963931723No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT121470.10792229807770903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG92650.049.1697733
ACCCGGC96200.047.354035
CGACCGT14100.047.3451541
CACCCGG96250.046.9887854
CGGCCCG112100.040.1288348
GCGATTT104450.038.0652894
CTCACCC132100.034.5929152
CCAGTAG60050.034.133575
ACCGTTG19600.034.0605853
TAGCATA61900.033.7165039
GACCGTT19700.033.6493452
CTACCGT41500.032.9605988
GTAGCAT64450.032.2366268
CCCGGCC146900.031.6117556
CGGACAC85600.031.21695312-13
CCGGCCC155950.029.780077
CGCAAAA38100.029.2435231
GACACGG93000.028.88374914-15
CTCGATT91700.028.78783248-49
CGGACAG94450.028.33959618-19
Produced by FastQC (version 0.11.5)