FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004828369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12008340
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT614760.5119441987818466No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA399860.33298524192352985No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC340360.28343634507350723No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT335810.2796473117849761No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG305280.2542233147962166No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC295790.24632047393728027No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG271310.2259346420904138No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC250980.20900474170451536No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG248970.2073309050210104No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA219860.18308941952010022No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC216720.18047457017372925No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG213030.17740170581445897No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC207200.17254674667772565No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT197060.16410261534899911No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC172350.14352524995128385No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT171580.14288402893322474No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA166140.13835384407836554No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC164480.13697147149397834No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA162400.13523934199064983No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT158870.1322997183624048No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT158820.13225808063395939No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA146950.12237328390102213No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC145610.12125739277868547No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT145380.12106585922783666No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC143500.11950028063828973No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT143440.11945031536415524No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC139620.11626919291092691No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA137860.11480354486964892No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG127940.10654261954608213No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC126230.10511860923324956No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT125770.1047355421315519No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT123570.10290348207995442No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)