FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004828372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11971532
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT612910.5119729037185884No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA399600.3337918655690851No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC338610.2828460050058756No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT330410.2759964221788824No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG305960.2555729709447379No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC293300.24499788331184347No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG275280.2299455073920364No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC257830.21536926101020318No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG247080.20638962498701086No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA220490.18417859969801692No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC213670.1784817515419079No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG212010.17709512867693125No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC206440.17244242424444925No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT198760.16602720520648484No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC170940.14278874249344192No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT169780.14181977711791607No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA167980.14031621015589318No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC166700.1392470069828991No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT161050.13452747735210496No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA159720.13341650843016584No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT155220.12965759102510854No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC151000.12613256181414376No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA147510.12321731253777711No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT144800.12095360894495374No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT144380.12060277665381505No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC142670.11917438803989332No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC139570.1165849116052983No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA137310.11469709975298066No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT129080.10782245747662036No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG127570.10656113185847893No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC126340.10553369443442995No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT124950.1043726066137567No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG119730.10001226242389027No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)