FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004828375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12005124
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT625300.5208609257180518No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA403240.3358899083424711No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC339780.28302914655442124No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT331610.27622371913859445No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG310890.25896442219172416No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC296740.247177788417679No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG277380.23105134107735997No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC258850.21561626518809804No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG252970.21071835659506724No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA221220.1842713161480048No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC214660.17880698275169835No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG214420.17860706811524812No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC211520.17619143292480777No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT198350.1652211172496011No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT169340.14105643556867883No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC169100.1408565209322286No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA168960.14073990406096595No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC167730.13971534154915852No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT162740.13555878306629737No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA160520.13370957267913267No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT155890.12985288615094687No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC149390.124438531413753No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT148160.12341396890194554No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC147870.1231724053829015No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT145130.12089004661676131No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA144890.12069013198031106No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA138920.11571725539861145No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC138840.11565061718646137No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT129620.10797056323616483No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG127230.10597974664818123No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT127120.10588811910647487No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC125310.10438042955657933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)