FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10673157
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCG25300.033.432053
CATTCGG30950.027.7844284
CGACCTA10600.024.8252224
CGACCGT12250.023.0698591
GCGATTT20400.022.11260294
CGCGAGT20100.021.0899831
TCGTAAG12150.020.1112046
ATTCGGG42400.019.838015
TCGGGCC45850.019.0624397
GCGGGTC38200.018.3716682
CCGTTGA15450.018.2487774
TTCGGGC47150.018.038816
ACCGTTG16550.017.6037273
TAGGTCG8900.017.4234813
GACGCTA38100.017.143317
CGCGGTC17800.016.9351751
CGGGCCG54850.016.0198968
GTCGTAA14700.015.9831965
GACCGTT17700.015.9662782
CTACCGT18650.015.910781
Produced by FastQC (version 0.11.5)