FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10634638
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTG4400.022.4277942
CCCGTAT16500.016.2903581
GTATCGA13450.016.0714654
CTCGTTT30700.015.6675651
TTCGCGC6600.014.9518617
CTCGCGT8850.014.9195061
CGTATCG18800.014.7473533
CTCGGAT32650.014.298541
CTCCGAT27950.013.497331
CGCTAAA6056.730261E-1113.250531
CCCGTCT36800.013.0704961
GTCCGAT11050.012.8026141
CTCGGTT25700.012.6606411
TCCGATT28400.012.5752082
CTCCGCT39500.012.5352181
CGCGTAA6401.72804E-1012.5258911
CTCATTT130650.012.5244821
CTCGACT26400.012.5035641
CTCGTTA8700.012.4666071
CCGTATC25350.012.234492
Produced by FastQC (version 0.11.5)