FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10580771
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA4900.018.2812961
CCCGTAT16650.018.1223771
GTATCGA14350.016.046614
CGTATCG19750.015.9421463
CTCGTTT29200.015.6616781
TTCGCGC7350.014.7038967
ACGTACG4750.014.34851452-53
CTCGGTT25850.014.2260231
ACCCGTT19400.014.0497782
CTCGCGT8100.013.9693331
CTCGGAT28950.013.6798121
CTCGTAT13900.013.5673371
ATGCGCG28500.013.27376984-85
CCGTATC24600.013.181272
TCGTACG3601.0859816E-513.0539122
TCGCGTG3751.65272E-512.5317562
TGTCGAG12800.012.4826875
CTCATAT58400.012.43246651
CTCGACT26350.012.3457611
CGTTTAC26200.012.1968245
Produced by FastQC (version 0.11.5)