FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14238472
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT24200.022.400191
CGCGTAA17250.021.0412181
GTATCGA21250.019.6800864
CGGCATG43450.018.1684763
GCGTAAC21200.017.2885172
ACCCGTT42300.016.8850462
CGTATCG37700.016.2032073
TTCGCGC11850.015.8602547
CGCGTAT4301.3442332E-915.3471891
GAGCGAC32050.015.1785554-55
CCGTATC43800.015.1266932
TCGACCG13950.014.65633630-31
ATCGAAG67550.014.3297236
ATTCGCA34300.014.11554790-91
ATGCGCG57500.013.68967984-85
CGAGCGA30300.013.65142252-53
CGTTTAC58800.013.5852475
GTTCGAC15050.013.58384428-29
CCCGTCT46650.013.4390711
ATCGCGT10900.013.36782694
Produced by FastQC (version 0.11.5)