FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16533944
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT273920.1656713002052021No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA233450.14119438169138593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT31750.026.4417691
CGCGTAA55050.025.959691
GCGTAAC60950.023.9003122
GTATCGA28600.023.4955484
TGTCGAG18750.021.3026315
CGTAACT69500.021.0952843
ACCCGTT73150.020.8135552
CGGCATG70050.020.0575833
TCGACCG27150.018.6082230-31
TTCGCGC18100.018.1733517
GTTCGAC27800.018.08784128-29
CGATCCG29000.017.17730382-83
CGTATCG66100.016.8485473
CCGTATC68950.016.8336492
CCGTTTA109000.016.7702164
CGTTTAC111950.016.6641045
CACGGAA87450.016.33948594
ACTAGTT96150.016.2257337
CGCGTAT3804.307367E-916.1351871
GTAACTA95100.016.0589964
Produced by FastQC (version 0.11.5)