FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16533944
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT445370.2693670669260764No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG290860.17591688952133866No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA230370.1393315472702702No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC171890.10396188592389087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG111000.043.977743
CACCCGG118900.040.9373364
GTCTAAC39450.040.1069221
ACCCGGC127750.038.4698375
GCGATTT111950.038.40588894
CGACCGT18250.038.043111
CCAGTAG89600.032.406345
TAGCATA93350.032.024159
CGACCTA20350.031.168154
GTAGCAT99900.030.0638058
ACGTGCG52000.029.3757238
TAACACG53200.029.143654
GCCAGTA100100.029.006554
CGGCCCG174350.028.3597458
CAGTAGC106550.028.0007616
CTCGATT108750.027.99705748-49
TGCGCGA49850.027.5329369
AACACGT57200.027.2704055
ATTCCGT110850.027.19160552-53
GACCGTT25250.027.1226482
Produced by FastQC (version 0.11.5)