FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11070148
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT421080.3803743183921299No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT358180.32355484316921507No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA248960.22489310892681832No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG230010.20777499993676687No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA219300.19810033253394627No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC214540.19380048035491484No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA200530.18114482299604304No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG187250.16914859674866137No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT186180.16818203333866902No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG166080.1500250945154482No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC142840.12903169858253025No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG141880.12816450150440625No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT135430.12233802113576078No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC133320.12043199422446746No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC116770.10548187792972596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC43950.058.2303661
TCACCCG90200.049.2268943
CACCCGG93600.047.5395664
ACCCGGC107050.041.611615
GCGATTT102750.038.41597794
CCAGTAG62650.038.4009325
ACGTGCG68650.037.5179258
TAACACG69750.036.6467744
AACACGT73100.035.096855
TAGCATA69850.034.9913949
GTAGCAT70700.034.5021978
GCCAGTA71900.033.7211154
CGACCGT11800.033.612931
ACACGTG77500.033.4074066
CAGTAGC73300.033.077956
CTCACCC138650.032.727482
CGACCTA9750.031.8068334
CAGACGT56050.031.7595861
TCAGACG42550.031.6267362
CATTCGG23800.031.390724
Produced by FastQC (version 0.11.5)