FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12311446
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT674810.5481159564847216No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG340680.27671810443712297No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC236530.19212203018231977No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT207570.16859920435016326No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC187940.15265469222705438No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC183790.14928384529323363No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA179580.14586426322302026No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC168750.13706757110415788No Hit
CGTGCCTCCTGGTCTCAGTATGGCGCTGTCCTGGGTTCTTACAGTCCTGA162490.13198287187386437No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT158690.12889631323566703No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT144410.11729735077423076No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG134790.10948348390595224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT16450.063.5786971
ACCCGGC120600.056.8481065
CACCCGG127100.053.9778184
TCACCCG128200.053.5146683
ACCGTTG19700.053.192043
CGGCCCG144000.047.902428
GCGATTT138750.046.39779794
CCAGTAG77500.044.9894035
CCCGGCC161600.042.976976
GACCGTT24800.042.5519182
TAGCATA85550.040.809639
GTAGCAT85950.040.565048
CCGGCCC172500.040.3427167
CGCAAAA48650.038.831671
CTCACCC192650.035.8498842
CAGTAGC97800.035.6026276
CGGACAC121900.035.17860812-13
AGTAGCA102850.033.899937
TAACCCC55850.033.4852689
GACACGG130850.033.18286514-15
Produced by FastQC (version 0.11.5)