FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13679132
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT27950.027.8627741
GTATCGA24650.026.8792344
CGGCATG45950.025.055053
TATCGAA34800.021.3349955
ACCCGTT34450.020.871262
CGCGTAA27850.020.6755391
CGTATCG41150.019.184663
CGTACGC13200.018.8675985
GCGTAAC31250.018.6473922
TCGACCG16050.018.00974830-31
CCGTATC44950.017.6686532
GCGAGCG42350.017.20331252-53
GAGCGAC42500.017.14184254-55
CGAGCGA35450.016.83919352-53
ATTCGCA43400.016.35164590-91
CCGTACG15700.016.162514
GTTCGAC18550.015.96311828-29
CGATCCG18400.015.83664582-83
CACCCGT47600.015.6665131
GCGACCA46700.015.60043356-57
Produced by FastQC (version 0.11.5)