FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14253076
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT176020.12349614918211338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT27000.026.02751
ACCCGTT31050.025.5759872
GTATCGA23850.023.8398934
CGTATCG41450.020.4058783
TATCGAA32000.020.117685
CGCGTAA13300.019.503881
CCGTATC45000.019.3181932
CACCCGT44300.018.8442781
CCCGTTT51450.017.1703763
TCGACCG10750.016.83084930-31
CGTTTAC54900.016.7761025
CGTACGA6750.016.71331454-55
CGCGTAT2002.9168473E-416.5073741
CCGTTTA55150.016.3592384
ATCGAAG60150.016.014956
TAATGCG16600.015.5693169
CGGCATG35100.015.2617753
GTTCGAC11400.015.25216928-29
ACGTACG7000.015.10865952-53
TTCGAAA35550.014.6720476
Produced by FastQC (version 0.11.5)