FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14214348
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG252620.17772183430432406No Hit
CAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGCTGC184380.12971400446928694No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG145700.10250206340804377No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT142370.10015936010571855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT17850.043.810891
GCGGGTC85850.035.9435422
ACCGTTG21700.034.6468053
GACCGTT23350.032.8867232
GCGATTT39300.032.28506594
CCGTTGA24200.031.4559354
CATTCGG30200.027.8515264
GGTCTGT114700.026.6287845
GTCTGTC118450.025.864546
ACCCGGC54700.025.8571435
CGCGGGT126350.025.3539661
GCATTCG33400.025.1831193
CTCGATT37950.024.45623648-49
ACGTGCG24400.024.2663848
TCACCCG59450.023.7912673
TGCAACC123550.023.54389694
GGGTCTG137800.022.7787654
GTCTAAC27100.022.5988461
CTACCGT24750.022.460481
TGCGCGA23750.021.9632179
Produced by FastQC (version 0.11.5)