FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13092763
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT28500.027.3052621
GTATCGA24850.027.2300384
CGTATCG37400.023.1184313
CCGTATC40650.022.0792852
TATCGAA32500.021.9772015
CGGCATG40350.020.4965743
ACCCGTT31950.020.0023142
CGCGTAA25350.019.1631361
TCGACCG17000.018.24502830-31
ATCGAAG63250.016.8645926
GTTCGAC18600.016.8015728-29
GCGTAAC29600.016.5102562
CGATCCG19250.016.11264482-83
ACGACTT21100.016.0937521
TAATGCG12050.015.5989999
CGAGCGA28600.015.20225952-53
GCGACCA37350.014.91388756-57
CCGTTTA48500.014.8238794
ATTCGCA35450.014.78348990-91
CTCGACT29900.014.6696261
Produced by FastQC (version 0.11.5)