FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13601630
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA156350.11494945826345812No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG155480.11430982904254856No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT148290.10902369789503169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA50050.028.0996671
CCCGTAT24750.026.3143621
GCGTAAC54900.026.1068292
GTATCGA22100.025.0902614
CGGCATG55450.024.4912953
ACCCGTT45000.024.1227112
CGTAACT66350.022.2384323
ACGACTT23100.021.4519271
TCGACCG23000.021.15006830-31
CCGTTTA62150.020.0364234
CGTATCG36800.019.9201323
TATCGAA29500.019.9114765
GTTCGAC24350.019.7850428-29
TGTCGAG10700.019.7626045
CGTTTAC64500.019.4521225
TGTCCGT28450.019.16157294
CGATCCG25150.018.50067982-83
CACGGAA77000.018.3709394
CCGTATC41500.018.3439792
GTAACTA81150.018.240534
Produced by FastQC (version 0.11.5)