FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13573069
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT512030.37723966480977883No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG279150.20566461424457505No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG182650.13456794480305082No Hit
CAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGCTGC178520.1315251547015638No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC157070.11572180175316282No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA139040.1024381442398915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG110500.049.788863
ACCCGGC116100.047.672355
CGACCGT15800.046.3218351
GCGATTT117750.043.1775894
CACCCGG131100.042.2881284
CGGCCCG145500.038.052638
CCAGTAG79150.035.4386335
TAGCATA80450.034.9972279
GTAGCAT83250.033.9306838
ACCGTTG22150.033.5135843
GCCAGTA86550.032.4075664
AGCGCTC50800.031.1816439
CTCGATT113250.031.17823448-49
CAGTAGC92050.030.6253456
ATTCCGT115950.030.1894452-53
CGGACAC120750.029.7242412-13
AGTAGCA96650.029.6078877
CCGGCCC190550.029.5913147
CGGACAG123500.028.95126518-19
CGTTCTT121600.028.62117676-77
Produced by FastQC (version 0.11.5)