FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15562188
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT3368772.1647148845650754No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1952901.2549006604983823No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC878610.5645799935073397No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT559380.3594481701416279No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA465860.2993537926672008No Hit
CGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTG296430.19048092723208332No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG287330.184633420441907No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC272330.17499467298557247No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC260030.16709090007137814No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA246300.15826823323301326No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGA232990.14971545132342573No Hit
CACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT221740.14248639073117483No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT200220.12865800104715353No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG194970.12528443943743642No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT190460.12238638936889852No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT189890.1220201169655578No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC188190.12092772558717321No Hit
CGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGG175610.11284402938712731No Hit
CCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATT170050.10927126699664597No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC169670.10902708539441883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT22400.067.490941
TCACCCG520400.065.058073
ACCCGGC538200.062.7924845
CACCCGG540300.062.5919084
ACCGTTG25600.059.288033
CTCACCC572700.059.160672
CCAGTAG332050.058.8413545
GACCGTT27100.055.7860452
TAGCATA358600.054.8767369
CCTCACC625850.054.5727731
GTAGCAT363050.054.191168
CAGTAGC361600.054.1757746
TGCCAGT367450.053.147363
GCCAGTA369350.052.8736274
CCTGCCA378700.052.0710221
GTCTAAC29100.049.524131
CGGCCCG685550.049.2880978
AGTAGCA398500.049.252797
CTGCCAG400100.048.9329382
GCGATTT677850.046.81085694
Produced by FastQC (version 0.11.5)