FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14090174
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT2165091.5365956445960143No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1289390.9150987063750952No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC616120.4372692629629698No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT341400.2422965110296012No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC279310.19823034122928504No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT236920.168145545966998No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT212210.15060850206675944No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA182700.12966482883745792No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC182490.12951578880431142No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT168330.11946623228357577No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT153500.10894116708565843No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC145740.10343378300367333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG342750.063.8912283
ACCCGGC350000.062.728895
CACCCGG359800.061.046444
CGACCGT17500.058.6293071
CGGCCCG382750.057.4093748
CCAGTAG228250.057.070975
CTCACCC415150.052.874932
GTAGCAT251800.051.9001738
TAGCATA253000.051.6166769
CAGTAGC255200.051.191396
GCCAGTA258050.050.6078034
AGTAGCA259700.050.303837
GGCCCGG447250.049.3400659
CCCGGCC449700.049.103836
GCGATTT426100.048.87972394
ACCGTTG21600.048.515883
CCGGCCC460650.048.282937
TGCCAGT276500.047.3158763
CCTCACC480650.046.2863541
CCTGCCA317750.041.6214331
Produced by FastQC (version 0.11.5)