FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14391296
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA167470.11636894967624875No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG166570.11574357166998718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT47400.033.919031
GTATCGA45300.033.713444
CGTATCG49400.032.6275983
CCGTATC56500.029.6918812
CGTACGC4000.029.3695935
TATCGAA61500.026.9722715
CGGCATG64700.026.2919143
ACGACTT21450.025.7173331
ATCGAAG72050.023.1532676
TCGACCG22300.022.66506630-31
GTTCGAC23500.021.90491128-29
TCGGCAT82350.021.6839262
TGTATTA83500.020.4282198
GTCGGCA96950.019.6472131
TCGAAGG87000.019.444367
GCGAGCG55350.019.4013452-53
ATTCGCA55250.019.30826290-91
CGATCCG26500.018.97663582-83
GCGACCA59400.018.43529156-57
CCGTACG6650.018.3725574
Produced by FastQC (version 0.11.5)