FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13730968
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG171080.12459427478091857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT39450.029.9955651
CGGCATG66400.028.874063
GTATCGA38950.028.834094
CGTATCG43550.027.6227933
ACGACTT29100.027.3794061
CCGTATC49350.024.8524462
TATCGAA53350.023.2533365
TCGACCG33400.022.38159830-31
TCGGCAT94050.022.2839322
GTTCGAC34450.021.76508928-29
TGTATTA90750.021.3337088
GTCGGCA105350.020.2271271
ATCGAAG61750.020.166236
CGACTTT50600.019.0379052
ATTCGCA60300.019.01637890-91
AAGTTCG39800.019.0108426-27
CGATCCG40400.018.72914782-83
GCGAGCG61200.018.698452-53
CATGTAT103100.018.4591676
GTTACGA9450.018.398644
Produced by FastQC (version 0.11.5)