FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13648851
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG172570.12643555124163933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT40500.033.6516881
GTATCGA40800.032.480164
CGTATCG45500.031.5005153
CCGTATC49850.029.0345272
ACGACTT27100.027.494881
TATCGAA54400.025.8286385
CGGCATG65550.024.8046233
ATCGAAG62450.023.2517286
GTTACGA7750.023.041524
GTTCGAC33350.022.26481428-29
TCGACCG33300.022.22768230-31
AAGTTCG38400.019.64304526-27
TCGGCAT88650.019.5073492
TGTATTA84300.019.4546018
CGACTTT49650.018.8348872
TCGAAGG78550.018.7250337
CGATCCG39800.018.4203882-83
CCCGTCG25600.018.2372461
ATTCGCA57650.018.05766990-91
GCGAGCG58750.017.92202652-53
Produced by FastQC (version 0.11.5)