FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13648851
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT498250.3650490433224013No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG312900.22925006654406296No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC263310.1929173378770125No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT256180.18769345492891673No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC220950.16188175839856409No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT210100.15393237130363574No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT196870.1442392476846586No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG179570.13156418807707695No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC159270.1166911412543078No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC144610.10595031039609122No Hit
CGTGCCTCCTGGTCTCAGTATGGCGCTGTCCTGGGTTCTTACAGTCCTGA144430.10581843116317996No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG138310.10133453724419732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT20350.057.5831381
ACCCGGC99600.049.022685
ACCGTTG24750.047.088243
TCACCCG106300.046.1091423
CGGCCCG109050.044.858058
GACCGTT26500.044.3975832
CACCCGG115850.042.3487364
GCGATTT114400.040.58408794
CGCAAAA60750.038.5008661
CCAGTAG80000.037.7125435
CCCGGCC149100.033.0914546
GTAGCAT94850.032.8962258
TAGCATA94550.032.2551089
CCGGCCC154650.032.1171657
CGGACAC99850.032.0251112-13
TAACCCC71550.031.9184639
AGTAGCA97200.031.8589387
CCTAGAG90350.030.88781
GGCCCGG161200.030.8123849
CGGACAG105600.030.37454418-19
Produced by FastQC (version 0.11.5)