FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12790807
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG204210.15965372630515026No Hit
CACATCTTATCGCATCCACTCCTGTCCACTCCCGTCCACTTTGCCCTCTT196920.15395432047407173No Hit
GTCAAAAGAAAGCTCTTTATTGATTGACTCAGCAATGCAGGGCTGGCACC181060.14155479009260322No Hit
GTCGGGTTGGAGGGGCAGGATGGCCCCCAAGTCTCTGTCCTGATCCAAGG142950.11175995384810357No Hit
CCACATCTTATCGCATCCACTCCTGTCCACTCCCGTCCACTTTGCCCTCT140060.10950051861465816No Hit
CTCCTGTCCACTCCCGTCCACTTTGCCCTCTTCCAGGCTGAAATCTTGCT137890.10780398766082545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAA8450.040.1734961
CCCGTAT63450.031.1348131
CGGTAAT11250.030.491482
GTATCGA64600.030.0414314
CGCTAGG5200.029.0141931
TATCGAA69950.027.8781175
TCGTATG9600.027.41357644-45
CGCTAGT1554.93892E-827.3762951
ACGACTT25500.026.6247861
CGTATCG86700.025.52763
CCGTATC87600.025.4262522
CGCGTAG2251.1277734E-1025.1456341
CCCGTCG31700.025.135721
CGCGATT1151.3491626E-424.598991
ATCGAAG101600.022.6623766
CACGGAA37350.022.64822694
TCGACCG25200.022.11101730-31
TATGCCG11150.021.9164748-49
GTTACGA5850.021.6875724
CGCGTAA39750.021.587291
Produced by FastQC (version 0.11.5)