FastQCFastQC Report
Wed 8 May 2024
EGAF00004828513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12790807
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC392770.30707210264371904No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT308180.24093866790422216No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC269670.21083110705993766No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG263900.20632005470804146No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC243520.1903867363490044No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG239160.18697803821134976No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA219620.17170144151186084No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT213550.16695584571012603No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT191310.1495683579620895No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC180510.1411247937678991No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT178280.13938135412409866No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA149320.11674009309967698No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT148730.11627882431499437No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG146100.1142226600714091No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA144690.11312030585716758No Hit
CTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACTATTCCTC144010.11258867403753338No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC142900.11172086327313047No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT136890.10702217616136338No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG133820.10462201485801483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)