FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11731053
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC261670.22305755502084937No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC232410.19811520756065118No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT220300.18779217858788977No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG182480.15555295845990977No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA181360.1545982274566486No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC172890.14737807424448598No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA163720.13956121415528513No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT153320.1306958548392885No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG144840.12346717724316819No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT144540.12321144572443753No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT143660.12246129993616088No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC129100.11004979689376562No Hit
CTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACTATTCCTC124300.10595809259407489No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC122770.10465386184854847No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA122760.10464533746459077No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT122680.10457714239292926No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT119800.10212211981311481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT20850.056.873291
ACCGTTG23500.050.7896923
GACCGTT25700.046.3234862
CGCAAAA59850.041.0412141
CCGTTGA32250.036.863844
TCGCATA5600.036.081817
GTCTAAC16450.034.612531
ACATGCC84300.031.0914842
GTAGGTC44350.029.7083342
TAACCCC84100.029.3899949
AGTAGGT46200.028.5187151
CCAGTAG51250.028.4235025
CACATGC93800.028.3940581
GTAGCAT52700.028.2656128
CCGTCAT79600.028.138941
GTTGACT72900.027.5622221
GCCAGTA55850.026.4189914
TAGCATA57550.026.0468449
GTGGCGA89550.026.0107862
GTAATCG8600.025.6808363
Produced by FastQC (version 0.11.5)