FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11729872
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA240790.20527930739568176No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG132460.11292535843528387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT41600.029.3583641
GTATCGA40350.028.7637254
TATCGAA43050.027.1780285
CTTCGCG21200.025.4890546
CGTATCG62300.022.4006253
TTCGCGC24400.022.1462277
CGGTAAT11650.021.8571381
CCGTATC64750.021.6981722
CGTTTAA18250.021.3701215
CCGGTAA9950.020.3784311
ACGACTT17450.020.2670841
ATCGAAG70050.020.2577196
ACACGCG14700.019.8182878
TGTCGAG14050.019.3973945
CGCTACT4750.018.8618981
GCGTTTA21250.018.132044
CCCGTCG20600.018.0836161
CGCTAGG5250.017.9637151
ACGTTCG7700.017.6969726
AGTTTAC166650.017.68276694
Produced by FastQC (version 0.11.5)