FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12554219
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA32250.031.1803914
CCCGTAT35900.030.4665891
CGTATCG36950.028.8675983
TATCGAA38900.026.8164235
CCGTATC41900.026.6908342
ATCGAAG58650.019.4686456
CGACTAT2753.0999217E-818.8577671
CGCGTAT1550.001013020618.2494541
TCGACCG7800.017.18036530-31
CCCGTCG16050.015.8616741
TCGAAGG74250.015.631287
CGCGTAA9400.015.5476281
CCGGTAA12500.015.0862141
TCGCGTG3454.266749E-714.9821042
CGCTAGT2257.163643E-414.6671531
GTTCGAC8750.014.50724928-29
CGGTAAT12750.014.3732062
TCAATCG46350.014.2943168
CTTCGCG9050.014.0188596
GCGTAAC11950.013.7625652
Produced by FastQC (version 0.11.5)