FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12554219
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT207230.16506801418710315No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC193090.15380486830761836No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC166790.13285573558976468No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA147490.1174824176637352No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT136600.10880804293759731No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG125830.1002292536078907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT19400.053.137331
ACCGTTG23800.042.845213
CCGTTGA25150.040.5453644
GACCGTT25250.040.4534572
CGCAAAA66000.035.3035661
TAACCCC71850.031.0652059
CGCGGGT45850.030.9018481
TCGCATA7700.029.2930627
GCGATTT25150.028.77447394
GTCGTAA7150.027.6034095
GTAATCG9550.026.5711213
GCGGGTC53300.026.405922
TTAACCC85450.026.1760438
CCGTCAT67050.025.9752831
ACATGCC86450.024.2299392
CGCGGTC10850.023.8610861
CACATGC93000.023.2320231
GTAGGTC43050.023.1803512
CCGTATG51000.022.9429174
ACCCGGC36250.022.4262965
Produced by FastQC (version 0.11.5)