FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12522711
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT34300.033.1322061
GTATCGA33600.031.8863494
CGTATCG37750.030.6215653
CCGTATC41550.028.9519732
TATCGAA39050.027.5564755
CGCGTAA8850.021.8458021
ATCGAAG57550.020.3312116
CGCTAGG6250.018.1075061
CCCGTCG14900.016.7732431
CGCTAGT2253.770585E-516.7662091
GCGTAAC11500.016.7531032
TCGAAGG71800.016.5579017
TCGACCG8500.015.48206830-31
CGGCATG28950.014.9330483
TCAATCG45800.014.5690788
GTTCGAC9450.014.17357828-29
CGCGTAG4352.5713234E-814.0922881
CGCATAT5853.8198777E-1113.7031531
CCGGTAA12650.013.4195951
TATCGCA27350.013.2294758
Produced by FastQC (version 0.11.5)