FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12019896
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA223610.18603322358196778No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT144160.11993448196223995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA22400.025.593644
CGCTAGT1501.1327465E-625.1452391
CCCGTAT26850.024.2321431
TATCGAA27300.021.6884295
TTACGAC8000.020.5588255
CTTCGCG19000.020.5279066
CCCGTCG19600.020.2059971
ACGACTT21650.020.034891
TTCGCGC20300.019.6759597
GTTACGA8650.019.013944
TCGACCG19550.018.51890830-31
ATCGCGT8450.018.35350494
CGATCCG19850.018.34879182-83
GTTCGAC20150.018.19797728-29
TACGACT9150.017.9749286
CGGCATG38100.017.7606773
CGCTATA2253.7758047E-516.7634931
AGACCGC8450.016.6834074
AAGTTCG21950.016.59270526-27
AGTTTAC163450.016.01518894
Produced by FastQC (version 0.11.5)