FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12019896
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC155410.12929396394111897No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG150440.12515915279133863No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT140480.1168728914127044No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC136250.11335372618864589No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT133410.11099097696019998No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC126970.10563319349851279No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC125770.10463484875409904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT15750.046.0209241
GTCTAAC28600.040.9777831
ACCGTTG18050.040.092753
GCGATTT40800.035.93657394
GACCGTT20200.035.416642
ACGTGCG35800.031.895768
ACCCGGC51550.031.8140625
TCACCCG54300.030.3759333
CACCCGG54200.030.1718774
CGCAAAA47000.029.942531
CCAGTAG52000.027.6528855
AACACGT42200.027.616055
CCGTTGA26900.027.426464
CTCGATT38150.027.09961548-49
GTAGCAT53600.027.0019828
TAACACG44000.026.1659054
CATTCGG18900.026.1065734
ATTCCGT40150.025.57413152-53
TAGCATA58800.025.4136339
GTGGCGA77800.025.3483432
Produced by FastQC (version 0.11.5)